BioTracker NIR-ASM NQO1 Live Cell Dye Live cell imaging fluorescent probe for near-infrared imaging of NQO1 enzyme in cancer cells in vitro and in vivo

NAD(P)H quinone oxidoreductase 1 (NQO1) is a cancer-specific target enzyme and accurate detection of NQO1 activity with high sensitivity and selectivity is useful for the early diagnosis of cancer. NQO1 is a two-electron reductase responsible for the detoxification of xenobiotics such as quinones and is abnormally overexpressed in many tumors and intimately linked with multiple carcinogenic processes. compared to healthy tissue, NQO1 expressed at levels of 5−200-fold above in tumors, such as the breast12, lung13,14, prostate15, stomach16, colon17, pancreas18, head, and neck cancer19. Consistent with this, NQO1 has been extensively explored to achieve highly selective and sensitive detection or visualization of tumor-associated events in different cancers.BioTracker NIR-ASM NQO1 Live Cell Dye demonstrated high selectivity for NQO1 over the other enzymes/substrates such as glutathione, aldehyde dehydrogenase 1, gamma-glutamyl transferase, glutathione peroxidase, apurinic/apyrimidinic endonuclease, cystathionine-beta-synthase, cathepsin L, glutathione S-transferase, NADH alone and the combination of nitroreductase and NQO1. Fluorescence only resulted from the specific cleavage of the probe by NQO1 in the presence of its co-factor NADH. NIR-ASM dye was not toxic to both normal and cancer cells even at higher concentrations (up to 100 muM). A high-intensity distribution of signals was obtained for NIR-ASM dye activation in each of the two NQO1-positive cancer cell lines (A549 and H460), while the negative normal cell lines IMR90 and HUVECs produced minimal fluorescence.Spectral properties:Excitation: 488 nmEmission: 644 nmResearch Category:Live Cell Dye