Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Ready Made Solution >=98% (HPLC), 2.54 mg/mL (10mM) in DMSO

Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP) is a proton (H+) ionophore and a potent mitochondrial uncoupling agent. This compound turns the mitochondrial membrane permeable to protons, thus dissipating the mitochondrial membrane potential and uncouples oxidative phosphorylation from ATP synthesis. FCCP is a potent tool for assaying mitochondrial respiration and investigating the role of mitochondria in cellular function.1,2FCCP induces mitophagy of SH-SY5Y neuroblastoma cell in a dose dependent manner. Low concentration of FCCP (1(mu)M) leads to a complete loss of mitochondrial membrane potential without triggering mitophagy, whereas at a high concentration (10 (mu)M), cytosol acidification is increased and mitochondrial degradation is attained.3 Additionally, FCCP (20 (mu)M) was able to induce apoptosis in lymphoid (CCRF-CEM) and myeloid (HL60) cells after 24hrs of incubation. 4 FCCP (0.5nM-5 (mu)M) reduces β-amyloid peptide production in K695sw cells without altering the β-amyloid precursor protein maturation or synthesis.5 Partial uncoupling of mitochondrial oxidative phosphorylation induced by FCCP (0.3 -3(mu)M) reduces micromotion and wound healing migration of human mesenchymal stem cells.6 Inhibition of F1F0-ATPase by FCCP (10 (mu)M), reduces the impairment of electromechanical activity in human myocardium.7