This HTRF kit enables the cell-based quantitative detection of phosphorylated eNOS (endothelial nitric oxide synthase) at Serine 1177..This HTRF cell-based assay conveniently and accurately detects phosphorylated eNOS at Serine 1177. eNOS is expressed primarily in endothelial cells, where its function is to control blood vessel dilation, blood pressure, and numerous other anti-atherosclerotic and vasoprotective effects. Serine 1177 is the most important phosphorylation site of eNOS, positively modulating its activity. Phosphorylation at serine 1177 is under the control of several kinases, including AKT, PKA, AMPK, CaMKK2b, and CHKI This HTRF cell-based assay conveniently and accurately detects phosphorylated eNOS at Serine 1177. .