TrueGel3D Hydrogel Kits FAST-DEXTRAN, allow cell recovery, CD cell-degradable crosslinker

The gel is formed by stable thioether bonds between maleimide groups of dextran (FAST-DEXTRAN) and thiol groups on CD cell-degradable crosslinker. Malemide-functionalized dextran is specifically used to decrease the time for gel formation. The dextran polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1UM) prior to the crosslinking step. The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.