Extra control lysate for the AlphaLISA SureFire Ultra Phospho-PDHA1 (Ser293) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-PDHA1 (Ser293) assay kit, but can also be ordered separately. This control lysate is intended for use with the AlphaLISA SureFire Ultra Human and Mouse Phospho-PDHA1 (Ser293) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HeLa cells were cultured to confluence in T175 flasks in 10% FBS containing medium and lysed in 8 mL of 1X SureFire Ultra Lysis Buffer.