Extra control lysate for the AlphaLISA SureFire Ultra ; Phospho-BTK (Tyr223) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-BTK (Tyr223) assay kit, but can also be ordered separately. This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-BTK (Tyr223) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. The lysate is prepared from U937 cells, cultured in a T175 flask to approximately 1X106 cells/mL in 10% FBS containing medium, then treated with 1% pervanadate for 30min and lysed with 5X SureFire Ultra Lysis buffer.