AlphaLISA Surefire Ultra Human Phospho-SLP76 (Tyr113), Control Lysate

Extra control lysate for the AlphaLISA SureFire Ultra Phospho-SLP76 (Tyr113) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-SLP76 (Tyr113) assay kit, but can also be ordered separately. This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-SLP76 (Tyr113) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate Jurkat cells were cultured in T175 flask to 106 cells/mL in 10% FBS containing medium, then treated with 11 mM H2O2 and 50 nM Calyculin A for 10 minutes and lysed at 3.2X106 cells/mL with 1X SureFire Ultra Lysis Buffer.